Studying the Proliferation of Human Peripheral Blood T Lymphocytes in Serum-Free Medium
نویسندگان
چکیده
منابع مشابه
Development of a chemically defined serum- and protein-free medium for growth of human peripheral lymphocytes.
A chemically defined, protein-free medium (designated CFBI 1000, where CFBI = Clayton Foundation Biochemical Institute) that supports human peripheral lymphocyte proliferation has been developed. This medium allows exploration of individual metabolic differences by varying the medium composition as well as providing a base to explore further the mechanisms of lymphocyte activation in a system i...
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Both phytohaemagglutinin and antibodies to the CD3 molecule induced proliferation and phosphoinositide hydrolysis in human peripheral-blood T lymphocytes, but the magnitude of the inositol phosphate response was small and the rate of accumulation slow [significant increases in Ins(1,4,5)P3 were observed only after 10 min]. Hence this response differs from the well-characterized Ins(1,4,5)P3 res...
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ژورنال
عنوان ژورنال: Bulletin of Experimental Biology and Medicine
سال: 2009
ISSN: 0007-4888,1573-8221
DOI: 10.1007/s10517-009-0535-x